Device

Part:BBa_K1256005

Designed by: Pei-Hong Chen   Group: iGEM14_Mingdao   (2014-06-09)

pSB1C3-Plac-SS-Magainin-MprF

Mingdao pSB1C3-Plac-SS-Magainin-MprF.png

MprF of Bacillus subtilis (DB2)added a RBS (BBa_B0034)was amplified by PCR and cloned into the vector of pSB1C3-Plac-SS-NB (Part:BBa_K1256003) using NheI and BamHI sites. Next, the cds of magainin was synthesized on the primers and subjeted to Gibson Assembly to make this plasmid. The [http://www.sigmaaldrich.com/catalog/product/sigma/m7402?lang=en&region=TW amino acid sequences] of magainin was obtained from Sigma-Aldrich, and the nucleotide sequence was optimized for engineering Escherichia coli using the [http://sg.idtdna.com/CodonOpt codon optimization] tool provided by Integrated DNA Technologies (IDT).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 362
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2888
    Illegal BamHI site found at 2957
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1283
    Illegal NgoMIV site found at 2339
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None